A label-free logic gate hairpin aptasensor for sensitive detection of ATP based on graphene oxide and PicoGreen dye

Abstract Background In this paper, a simple, enzyme-free, label-free fluorescence, high sensitivity logic gate hairpin aptasensor was developed for adenosine triphosphate (ATP) detection based on graphene oxide (GO) and PicoGreen dye. Methods Using single-strand deoxyribonucleic acid (DNA) as input signal fluorescence output signal, if DNA (DNA-L), (DNA-S), ATP were present at the same time, one segment of DNA-L formed ring with ATP, other completely complementary stem DNA-S. The detached from GO surface, dye embedded into stem, enhanced. molecular constructed through establishment histogram, circuit, truth table, formula. biosensor-related performances including sensitivity, selectivity, linearity investigated, respectively. Results We have successfully AND gate. limit is 138.0 pmol/L (3?/slope) range 50–500 nmol/L ( R 2 = 0.98951), its 4.748 × 10 6 –6.875 8 a.u. (mol/L) ?1 . Conclusions aptamer sensor has advantages low limit, cost, can be applied to in actual human urine samples.